Phenotype of blood group systems MNS, Duffy, Kidd, Lutheran, Lewis, Kell and P1PK in donors from Khanty-Mansiysk

Zinovieva A.V.¹, Rokina O.A.¹, Sukhareva A.S.¹, Kutefa E.I.¹, Zhiburt E.B. ²

Rationale: Antigens of the blood group systems MNSs, Duffy, Kidd, Lutheran, Lewis, Kell (Kp^a, Kp^b) and P1PK are immunogenic, cause the production of irregular antibodies and corresponding difficulties in the selection of donor red blood cells.

Objective: to create a cohort of regular donors, phenotyped according to the antigens of the above blood group systems.

Methods: From 2014 to 2023 in the blood transfusion department of the Okrug Clinical Hospital (Khanty-Mansiysk). We examined 830 regular donors with a donation frequency of more than 3 times a year. Along with the ABO and Rh phenotypes, antigens of the MNSs, Duffy, Kidd, Lutheran, Lewis, Kell (Kp^a, Kp^b) and P1PK blood group systems were determined. Red blood cells were phenotyped in gel cards using an IH-100 analyzer (Biorad, Switzerland). The prevalence of phenotypes was compared with literature data on the prevalence of similar phenotypes in Caucasians using the chi-square method at a significance level of 0.05.

Results: In Khanty-Mansiysk, the M+N-S-s+ phenotype is 9.2% more common (p<0.01) and the M+N+S+s+ phenotype is 11.1% less common (p<0.01). The Le (a-b-) phenotype among donors is 9.1% more common than in the comparison group (p<0.001). These donors should be aware of the increased risk of coronary artery disease in individuals with the Lewis null phenotype. Fy^a and Fy^b antigens were detected in 71% and 76% of cases, respectively. The Duffy null phenotype, Fy(a-b-), was found in 4 donors. The frequency of occurrence of Jk^a and Jk^b antigens was 82.4% and 64.3%, respectively. 41 donors with the rare Kidd null phenotype were identified. For the Lutheran blood group system, the frequencies of Lu^a and Lu^b antigens were 2.0% and 98%, respectively. Phenotype P1 among donors is 16% less common than in the comparison group (p<0.001).

Conclusion: For the first time, the frequency of phenotypes of six additional blood group systems in donors from Khanty-Mansiysk was studied. Compared to literature data, the following phenotypes are more common in Khanty-Mansiysk: M+N-S-s+, Le(a-b-), Fy(a-b-), Jk(a-b-) and less commonly – phenotypes M+N+S+s+ and P1. The effectiveness of selecting blood from donors with a known extended erythrocyte phenotype for the prevention of transfusion reactions caused by irregular antibodies remains to be assessed.

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